Era T, Asou N, Yamaguchi K, Yamasaki H, Kamada N, Nishikawa S, Takatsuki K
Second Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
Leukemia. 1995 Oct;9 Suppl 1:S26-8.
Recently, two genes, AML1 and ETO have been isolated from the chromosomal breakpoint of t(8;21). In this study, we isolated and identified fusion transcripts from a leukemic cell line carrying t(8;21). We demonstrated by PCR analysis that these transcripts are consistently expressed in fresh leukemic cells with t(8;21). On the other hand, the wild type of ETO is expressed in several hematopoietic cells from different lineage, while the expression of AML1 was present in all hematopoietic cells investigated. These widespread expression suggests these molecules play an essential role in hematopoiesis.
最近，从t(8;21)的染色体断点处分离出了两个基因，AML1和ETO。在本研究中，我们从携带t(8;21)的白血病细胞系中分离并鉴定了融合转录本。我们通过PCR分析证明，这些转录本在携带t(8;21)的新鲜白血病细胞中持续表达。另一方面，ETO的野生型在来自不同谱系的几种造血细胞中表达，而AML1的表达则存在于所有研究的造血细胞中。这些广泛的表达表明这些分子在造血过程中起着至关重要的作用。
