Tempel C, Schiffenbauer Y S, Meir G, Neeman M
Department of Hormone Research, Weizmann Institute of Science, Rehovot, Israel.
Magn Reson Med. 1995 Aug;34(2):213-8. doi: 10.1002/mrm.1910340212.
The preovulatory rat follicle reaches a diameter of 1 mm with no internal blood vessels. Nutrient supply to the enclosed oocyte depends solely on passive diffusion across the follicular wall and the follicular fluid. Spin-echo and stimulated-echo NMR microscopy experiments were applied here for studying modulations in water diffusion during gonadotropin-induced maturation of perfused rat ovarian follicles (32 degrees C). Two diffusion compartments were observed for the follicular wall. The intracellular water diffusion coefficient, measured at a short diffusion time (9 ms) was 0.28 x 10(-5) cm2/s. Diffusion at long diffusion times was restricted to 16 microns, the size of cells in the follicular wall, and did not change during maturation. In the follicular fluid a transient 26% decrease in the diffusion coefficient was observed 4-7 h after gonadotropin stimulation, a change that is bound to affect the metabolic balance of the oocyte before ovulation.
排卵前的大鼠卵泡直径达1毫米，内部无血管。封闭在其中的卵母细胞的营养供应完全依赖于穿过卵泡壁和卵泡液的被动扩散。本文应用自旋回波和受激回波核磁共振显微镜实验，研究促性腺激素诱导的灌流大鼠卵巢卵泡（32℃）成熟过程中水分扩散的变化。在卵泡壁观察到两个扩散区室。在短扩散时间（9毫秒）测量的细胞内水扩散系数为0.28×10⁻⁵平方厘米/秒。长扩散时间的扩散限制在16微米，即卵泡壁细胞的大小，且在成熟过程中不变。在卵泡液中，促性腺激素刺激后4至7小时观察到扩散系数短暂下降26%，这一变化必然会影响排卵前卵母细胞的代谢平衡。
