Grasso G M, Sammarco M L, Ripabelli G, Ruberto A, Iannitto G
Department of Animal, Plant and Environmental Sciences, Faculty of Agriculture, University of Molise, Campobasso, Italy.
Microbios. 1995;82(333):245-9.
Urease activity is a feature of gastric helicobacters, and its abundant production provides an indirect means of detecting their colonization. A method for mapping urease-positive areas directly on the gastric mucosa was developed, and 57.8% of pigs had evidence of Helicobacter colonization based on urease assay. Moreover, 89.2% of urease-positive pigs had gastritis, confirming that the known association found in man between Helicobacter and gastritis was found also in pigs. The proposed urease assay allowed detection of all urease-positive areas on the gastric mucosa, thus overcoming the biopsy sampling problems derived from the patchy distribution of helicobacters. In this way, gastric mucosa specimens from urease-positive areas could be usefully utilized for culture attempts and for microscopic examination.
脲酶活性是胃内螺杆菌的一个特征，其大量产生为检测它们的定植提供了一种间接方法。开发了一种直接在胃黏膜上绘制脲酶阳性区域的方法，基于脲酶检测，57.8%的猪有幽门螺杆菌定植的证据。此外，89.2%的脲酶阳性猪患有胃炎，证实了在人类中发现的幽门螺杆菌与胃炎之间的已知关联在猪中也存在。所提出的脲酶检测方法能够检测胃黏膜上所有脲酶阳性区域，从而克服了因螺杆菌呈斑片状分布而导致的活检采样问题。通过这种方式，来自脲酶阳性区域的胃黏膜标本可有效地用于培养尝试和显微镜检查。
