Chumakov V N, Piotukh K V, Volodin A A, Kostrov S V
Mol Biol (Mosk). 1995 Jul-Aug;29(4):756-60.
A set of different hybrid genes encoding functionally active enzymes was obtained by homologous recombination between the fragments of related Bacillus amyloliquefaciens and Bacillus brevis metalloprotease genes cloned in plasmid vector in tandem orientation. The nucleotide sequences of hybrid genes were analyzed. It was demonstrated that the presence even of short homologous regions is sufficient for effective recombination in Bacillus cells.
