Cambier A, Peltier A P
Biomedicine. 1978 Dec;28(6):332-6.
Lymphokine preparations have been obtained from normal guinea pig spleen cells stimulated in vitro by optimal doses of insoluble Concanavalin A. The biological activity of these preparations was estimated by Macrophage Inhibition Tests performed with peritoneal cells from identical guinea pigs. Addition of these preparations to culture of normal adherent guinea pig spleen cells, gives rise to a dose related inhibition of the normal synthesis of C2, the second component of the complement system, by these latter cells. This inhibition of synthesis, appears to be due to a functional effect of lymphokine on C2-producing cells and not to a cytotoxic effect. Preliminary results indicate that the same lymphokine preparations can have a stimulatory rather than an inhibitory effect on synthesis of haemolytic C1 by the same cells.
已从经最佳剂量不溶性伴刀豆球蛋白A体外刺激的正常豚鼠脾细胞中获得淋巴细胞因子制剂。这些制剂的生物活性通过用来自相同豚鼠的腹腔细胞进行的巨噬细胞抑制试验来评估。将这些制剂添加到正常贴壁豚鼠脾细胞培养物中，会导致这些细胞对补体系统第二成分C2的正常合成产生剂量相关的抑制作用。这种合成抑制似乎是由于淋巴细胞因子对产生C2的细胞的功能作用，而非细胞毒性作用。初步结果表明，相同的淋巴细胞因子制剂对相同细胞的溶血C1合成可能具有刺激而非抑制作用。
