Bregman B S, Kunkel-Bagden E, Schnell L, Dai H N, Gao D, Schwab M E
Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA.
Nature. 1995 Nov 30;378(6556):498-501. doi: 10.1038/378498a0.
There is little axonal growth after central nervous system (CNS) injury in adult mammals. The administration of antibodies (IN-1) to neutralize the myelin-associated neurite growth inhibitory proteins leads to long-distance regrowth of a proportion of CNS axons after injury. Our aim was: to determine if spinal cord lesion in adult rats, followed by treatment with antibodies to neurite growth inhibitors, can lead to regeneration and anatomical plasticity of other spinally projecting pathways; to determine if the anatomical projections persist at long survival intervals; and to determine whether this fibre growth is associated with recovery of function. We report here that brain stem-spinal as well as corticospinal axons undergo regeneration and anatomical plasticity after application of IN-1 antibodies. There is a recovery of specific reflex and locomotor functions after spinal cord injury in these adult rats. Removal of the sensorimotor cortex in IN-1-treated rats 2-3 months later abolished the recovered contact-placing responses, suggesting that the recovery was dependent upon the regrowth of these pathways.
成年哺乳动物中枢神经系统（CNS）损伤后轴突生长很少。给予抗体（IN-1）以中和髓磷脂相关的神经突生长抑制蛋白，可导致损伤后一部分CNS轴突进行长距离再生。我们的目的是：确定成年大鼠脊髓损伤后，用针对神经突生长抑制剂的抗体治疗，是否能导致其他脊髓投射通路的再生和解剖可塑性；确定解剖投射在长时间存活期是否持续存在；以及确定这种纤维生长是否与功能恢复相关。我们在此报告，应用IN-1抗体后，脑干-脊髓以及皮质脊髓轴突会发生再生和解剖可塑性。这些成年大鼠脊髓损伤后特定反射和运动功能会有所恢复。在2 - 3个月后对接受IN-1治疗的大鼠切除感觉运动皮层，消除了恢复的接触放置反应，这表明恢复依赖于这些通路的再生。
