Przewłocki R, Kamińska B, Lukasiuk K, Nowicka D Z, Przewłocka B, Kaczmarek L, Lasoń W
Neuropeptide Research Department, Institute of Pharmacology, Kraków, Poland.
Neuroscience. 1995 Sep;68(1):73-81. doi: 10.1016/0306-4522(95)00144-8.
An in situ hybridization study showed that limbic seizures induced by kainate strongly augmented the prodynorphin and proenkephalin messenger RNA levels in granular cells of the rat hippocampal dentate gyrus. Pentylenetetrazole increased the level of proenkephalin messenger RNA, but slightly decreased that of prodynorphin messenger RNA in the dentate gyrus. Administration of kainate to rats caused a profound increase in messenger RNAs of the transcription factor genes c-fos and c-jun in the dentate gyrus, followed by an increase in the level of the transcriptional complex activator protein-1 in hippocampal neurons. Pentylenetetrazole also elevated the formation of activator protein-1, but the effect appeared earlier than that induced by kainate. Thus, recurrent limbic seizures activate both prodynorphin and proenkephalin genes, whereas generalized clonic-tonic seizures seem to decrease the prodynorphin and increase the proenkephalin gene expression in the dentate gyrus. Furthermore, our present results suggest that the transcription factors, c-fos, c-jun and activator protein-1 complex may be involved in the process of inducing the hippocampal proenkephalin gene, while these factors might be differently involved in regulation of prodynorphin gene expression.
原位杂交研究表明，红藻氨酸诱发的边缘叶癫痫可显著提高大鼠海马齿状回颗粒细胞中前强啡肽原和前脑啡肽原信使核糖核酸水平。戊四氮可提高前脑啡肽原信使核糖核酸水平，但使齿状回中前强啡肽原信使核糖核酸水平略有下降。给大鼠注射红藻氨酸会使齿状回中转录因子基因c-fos和c-jun的信使核糖核酸显著增加，随后海马神经元中转录复合物激活蛋白-1水平升高。戊四氮也会提高激活蛋白-1的形成，但该效应比红藻氨酸诱导的效应出现得更早。因此，反复出现的边缘叶癫痫会激活前强啡肽原和前脑啡肽原基因，而全身性阵挛-强直癫痫似乎会降低齿状回中前强啡肽原基因的表达并增加前脑啡肽原基因的表达。此外，我们目前的结果表明，转录因子c-fos、c-jun和激活蛋白-1复合物可能参与诱导海马前脑啡肽原基因的过程，而这些因子可能以不同方式参与前强啡肽原基因表达的调控。
