中脑x神经母细胞瘤杂交细胞系MES23.5中电压门控Ca2+通道的α-1亚基。

Alpha-1 subunits of voltage gated Ca2+ channels in the mesencephalon x neuroblastoma hybrid cell line MES23.5.

作者信息

Schneider T, Perez-Reyes E, Nyormoi O, Wei X, Crawford G D, Smith R G, Appel S H, Birnbaumer L

机构信息

Department of Neurology, Baylor College of Medicine, Houston, TX 77030, USA.

出版信息

Neuroscience. 1995 Sep;68(2):479-85. doi: 10.1016/0306-4522(95)00147-b.

DOI:10.1016/0306-4522(95)00147-b

PMID:7477958

Abstract

The identity of alpha 1 subunits from voltage operated Ca2+ channels was determined in the rat/mouse mesencephalon x N18TG2 hybridoma cell line MES23.5, by sequence analysis of reverse transcription-polymerase chain reaction products and antagonist binding. Sequences were derived from the L-(alpha 1D), Q-(alpha 1A) and omega-conotoxin GVIA sensitive N-type (alpha 1B) Ca2+ channel alpha 1 subunits. The amplified fragments, which are homologous to the region between domain III and IV of known alpha 1 subunits, reveal splice variation in the L- and Q-type alpha 1 subunit of MES23.5 cells. The transcripts of alpha 1 subunits in these cells were quantified by RNAase protection assay. The data show the existence of different Ca2+ channel types in a single cell line and may reflect multiple functions of voltage operated Ca2+ channels during growth, differentiation and transmitter release.

摘要

通过逆转录-聚合酶链反应产物的序列分析和拮抗剂结合，在大鼠/小鼠中脑x N18TG2杂交瘤细胞系MES23.5中确定了电压门控Ca2+通道α1亚基的身份。序列来自L型（α1D）、Q型（α1A）和ω-芋螺毒素GVIA敏感的N型（α1B）Ca2+通道α1亚基。扩增片段与已知α1亚基结构域III和IV之间的区域同源，揭示了MES23.5细胞L型和Q型α1亚基的剪接变异。通过核糖核酸酶保护试验对这些细胞中α1亚基的转录本进行定量。数据表明在单一细胞系中存在不同类型的Ca2+通道，这可能反映了电压门控Ca2+通道在生长、分化和递质释放过程中的多种功能。