新生大鼠脑内X射线诱导凋亡后的阿米巴样小胶质细胞反应。

Amoeboid microglial response following X-ray-induced apoptosis in the neonatal rat brain.

作者信息

Ferrer I, Olivé M, Blanco R, Ballabriga J, Cinós C, Planas A M

机构信息

Unitat de Neuropatologia, Hospital Prínceps d'Espanya, Universitat de Barcelona, Spain.

出版信息

Neurosci Lett. 1995 Jun 30;193(2):109-12. doi: 10.1016/0304-3940(95)11679-q.

DOI:10.1016/0304-3940(95)11679-q

PMID:7478153

Abstract

The phagocytic response following X-ray-induced apoptosis in the neonatal rat brain was examined by immunohistochemistry with the antibodies OX-6 and OX-42 which recognize MHC class II antigens and the CR3 complement receptor, respectively. Few OX-6-immunoreactive cells were observed in control rats, and in rats irradiated at postnatal day 2 and examined during the first 2 postnatal weeks. However, a transient increase in the number of OX-42-immunoreactive amoeboid microglia, containing large numbers of apoptotic cells, occurred at 6, 24 and 48 h after irradiation when compared with age-matched controls. These results show that X-ray-induced apoptosis promotes a short-lasting phagocytic response.

摘要

通过分别使用识别MHC II类抗原的抗体OX-6和识别CR3补体受体的抗体OX-42进行免疫组织化学，研究了新生大鼠脑内X射线诱导凋亡后的吞噬反应。在对照大鼠以及出生后第2天接受照射并在出生后前2周内进行检查的大鼠中，很少观察到OX-6免疫反应性细胞。然而，与年龄匹配的对照相比，照射后6、24和48小时出现了OX-42免疫反应性阿米巴样小胶质细胞数量的短暂增加，这些细胞含有大量凋亡细胞。这些结果表明，X射线诱导的凋亡促进了短暂的吞噬反应。