Cubells J F, Baker H, Volpe B T, Smith G P, Das S S, Joh T H
Laboratory of Molecular Neurobiology, Burke Medical Research Institute, White Plains, NY 10605, USA.
Neurosci Lett. 1995 Jul 7;193(3):189-92. doi: 10.1016/0304-3940(95)11699-w.
To determine whether a trans-synaptic mechanism triggered the effects of reserpine on adrenomedullary mRNAs encoding the norepinephrine transporter and tyrosine hydroxylase, we administered 10 mg/kg reserpine to rats after unilateral splanchnicotomy, and examined their adrenal medullas using quantitative in situ hybridization. Splanchnicotomy did not alter the decrease in norepinephrine transporter mRNA that follows reserpine administration, but diminished the reserpine-induced increase in tyrosine hydroxylase mRNA by almost 80%. Despite the latter effect, reserpine still induced a significant increase in tyrosine hydroxylase mRNA in denervated adrenal medullas, compared to vehicle-treated adrenal medullas. These results show that a trans-synaptic mechanism does not trigger the decrease in adrenomedullary norepinephrine transporter mRNA following reserpine. In addition, an innervation-independent mechanism mediates a portion of the reserpine-induced increase in adrenomedullary tyrosine hydroxylase mRNA.
为了确定跨突触机制是否触发了利血平对编码去甲肾上腺素转运体和酪氨酸羟化酶的肾上腺髓质mRNA的影响，我们在大鼠单侧内脏神经切断术后给予其10mg/kg利血平，并使用定量原位杂交技术检查其肾上腺髓质。内脏神经切断术并未改变利血平给药后去甲肾上腺素转运体mRNA的减少，但使利血平诱导的酪氨酸羟化酶mRNA增加减少了近80%。尽管有后一种效应，但与用赋形剂处理的肾上腺髓质相比，利血平仍能诱导去神经支配的肾上腺髓质中酪氨酸羟化酶mRNA显著增加。这些结果表明，跨突触机制不会触发利血平给药后肾上腺髓质去甲肾上腺素转运体mRNA的减少。此外，一种不依赖神经支配的机制介导了利血平诱导的肾上腺髓质酪氨酸羟化酶mRNA增加的一部分。
