Singh V, Schweitzer J B
Department of Pathology, College of Medicine, University of Tennessee, Memphis 38103, USA.
Neurosci Lett. 1995 Jul 14;194(1-2):117-20. doi: 10.1016/0304-3940(95)11744-h.
Cholinergic neurons of the basal forebrain express the p75 (low affinity) nerve growth factor receptor (NGFr) on the cell surface. It has been previously shown that an immunotoxin that recognizes the p75 NGFr, IgG 192-saporin, eliminates these neurons as judged by a variety of techniques after intraventricular injection into rat brain. Here we show that this loss of neurons can also be identified by detecting the disappearance of the p75 NGFr mRNA utilizing a non-radioactive in situ hybridization method.
基底前脑的胆碱能神经元在细胞表面表达p75（低亲和力）神经生长因子受体（NGFr）。先前的研究表明，一种识别p75 NGFr的免疫毒素IgG 192 - 皂草素，在脑室内注射到大鼠脑内后，通过多种技术判断，能消除这些神经元。在此我们表明，利用非放射性原位杂交方法检测p75 NGFr mRNA的消失，也可识别神经元的这种缺失。
