Sakai M, Fujii T, Karasawa N, Arai R, Nagatsu I
Department of Anatomy, School of Medicine, Fujita Health University, Aichi, Japan.
Neurosci Lett. 1995 Jul 14;194(1-2):142-4. doi: 10.1016/0304-3940(95)11716-a.
We examined by immunohistochemistry the effect of salt loading on the expression of tyrosine hydroxylase (TH), aromatic L-amino acid decarboxylase (AADC), and GTP cyclohydrolase I (GCH) in Purkinje cells of the mouse cerebellum. In control mice, only a few Purkinje cells were positive for TH or AADC. No Purkinje cells were stained for GCH. Drinking 2% sodium chloride for 2 weeks resulted in an increase in the number of TH- or AADC-positive Purkinje cells in the caudal vermis, paraflocculus and flocculus of the cerebellum. In contrast, no Purkinje cells were immunoreactive to GCH or L-DOPA after the salt loading. The present findings suggest that the salt loading differentially affects the expression of TH, AADC and GCH in Purkinje cells of the mouse cerebellum.
我们通过免疫组织化学方法检测了盐负荷对小鼠小脑浦肯野细胞中酪氨酸羟化酶（TH）、芳香族L-氨基酸脱羧酶（AADC）和GTP环化水解酶I（GCH）表达的影响。在对照小鼠中，只有少数浦肯野细胞TH或AADC呈阳性。没有浦肯野细胞被GCH染色。饮用2%氯化钠溶液2周导致小脑蚓部尾侧、旁绒球和绒球中TH或AADC阳性浦肯野细胞数量增加。相比之下，盐负荷后没有浦肯野细胞对GCH或左旋多巴呈免疫反应性。目前的研究结果表明，盐负荷对小鼠小脑浦肯野细胞中TH、AADC和GCH的表达有不同影响。
