碱性成纤维细胞生长因子对人小胶质细胞中诱导型一氧化氮合酶mRNA表达的抑制作用。
Inhibition of inducible nitric oxide synthase mRNA expression by basic fibroblast growth factor in human microglial cells.
作者信息
Colasanti M, Di Pucchio T, Persichini T, Sogos V, Presta M, Lauro G M
机构信息
Department of Biology, III University of Rome, Italy.
出版信息
Neurosci Lett. 1995 Jul 28;195(1):45-8. doi: 10.1016/0304-3940(95)11778-u.
The effect of basic fibroblast growth factor (bFGF) on inducible nitric oxide synthase (iNOS) mRNA expression in human cultured ramified microglial cells was investigated. Using RT-PCR and Southern blot analysis, we found that bFGF prevented the iNOS gene expression as induced by LPS/TNF alpha. Also, bFGF dose-dependently inhibited nitrite levels in treated cell supernatants. That the early presence of bFGF during LPS/TNF alpha induction was essential indicates that iNOS gene expression can be transcriptionally regulated.
研究了碱性成纤维细胞生长因子（bFGF）对人培养的分枝状小胶质细胞中诱导型一氧化氮合酶（iNOS）mRNA表达的影响。通过逆转录聚合酶链反应（RT-PCR）和Southern印迹分析，我们发现bFGF可阻止脂多糖（LPS）/肿瘤坏死因子α（TNFα）诱导的iNOS基因表达。此外，bFGF以剂量依赖的方式抑制处理后的细胞上清液中的亚硝酸盐水平。LPS/TNFα诱导过程中bFGF的早期存在至关重要，这表明iNOS基因表达可受到转录调控。
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