Uchihara T, Duyckaerts C, He Y, Kobayashi K, Seilhean D, Amouyel P, Hauw J J
Laboratoire de Neuropathologie, Université Pierre et Marie Curie, INSERM U360, Hôpital de la Salpêtrière, Paris, France.
Neurosci Lett. 1995 Jul 28;195(1):5-8. doi: 10.1016/0304-3940(95)11763-m.
The spatial relationship of apolipoprotein E (apoE)-like immunoreactivity (IR) to amyloid beta-peptide (A beta), astrocytes and microglial cells in the brain of Alzheimer's disease was studied by double immunolabelling. Diffuse apoE-like IR was seen in A beta diffuse deposits, and markedly increased in the core of classic senile plaques. Microglial cells, sometimes immunoreactive for apoE, were frequent in areas of apoE-like IR, where they often grouped into clusters in the core of apoE-labelled senile plaques. Although astrocytic processes were seen within these senile plaques, the cell bodies were always at a distance from the core. None of these astrocytes expressed apoE-like IR. Microglial cells, some of them immunoreactive for apoE, were seen in the center of apoE-labelled senile plaques. These data suggest that microglial cells play a more significant role than astrocytes in apoE deposition in senile plaques of Alzheimer disease.
通过双重免疫标记研究了阿尔茨海默病大脑中载脂蛋白E（apoE）样免疫反应性（IR）与β淀粉样肽（Aβ）、星形胶质细胞和小胶质细胞的空间关系。在Aβ弥漫性沉积物中可见弥漫性apoE样IR，在经典老年斑的核心中明显增加。小胶质细胞有时对apoE呈免疫反应性，在apoE样IR区域很常见，它们经常在apoE标记的老年斑核心中聚集成簇。尽管在这些老年斑内可见星形胶质细胞突起，但细胞体总是与核心有一定距离。这些星形胶质细胞均未表达apoE样IR。在apoE标记的老年斑中心可见小胶质细胞，其中一些对apoE呈免疫反应性。这些数据表明，在阿尔茨海默病老年斑中apoE沉积过程中，小胶质细胞比星形胶质细胞发挥更重要的作用。
