Pan M G, Wang Y H, Hirsch D D, Labudda K, Stork P J
Vollum Institute for Advanced Biomedical Research, Department of Cell Biology and Anatomy, Oregon Health Sciences University, Portland 97201, USA.
Oncogene. 1995 Nov 16;11(10):2005-12.
PC12/Wnt-1 cells display morphological changes in response to stimulation by select growth factors but do not respond to NGF. Furthermore, stimulation by EGF can induce neuronal differentiation in these cells but not in wild type cells. We have found that in these cells, compared to wild type PC12 cells, FGF and EGF stimulation of MAP kinase activity is enhanced, while NGF stimulation of MAP kinase in diminished. Finally, in cells expressing Wnt-1, the effect of cyclic adenosine monophosphate (cAMP) on MAP kinase activation is reversed; cAMP stimulates MAP kinase in wild type PC12 cells but inhibits MAP kinase in PC12/Wnt-1 cells. These data suggest that Wnt-1 expression alters the specificity of growth factor signaling in neuronal cells.
PC12/Wnt-1细胞在受到特定生长因子刺激时会表现出形态变化，但对神经生长因子（NGF）无反应。此外，表皮生长因子（EGF）刺激可诱导这些细胞发生神经元分化，而野生型细胞则不会。我们发现，与野生型PC12细胞相比，在这些细胞中，成纤维细胞生长因子（FGF）和EGF对丝裂原活化蛋白激酶（MAP激酶）活性的刺激增强，而NGF对MAP激酶的刺激减弱。最后，在表达Wnt-1的细胞中，环磷酸腺苷（cAMP）对MAP激酶激活的作用发生了逆转；cAMP在野生型PC12细胞中刺激MAP激酶，但在PC12/Wnt-1细胞中抑制MAP激酶。这些数据表明，Wnt-1的表达改变了神经元细胞中生长因子信号传导的特异性。
