Perrem K, Rayner J, Voss T, Sturzbecher H, Jackson P, Braithwaite A
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, ACT, Australia.
Oncogene. 1995 Oct 5;11(7):1299-307.
There is now much evidence to suggest that the p53 tumour suppressor protein functions to monitor the integrity of the genome. When DNA damage is detected, p53 suppresses cell growth to allow repair or directs the cell into apoptosis. The mechanism of action of p53 is as yet unclear but recent evidence has accumulated to suggest that p53 might act by regulating gene expression. Consistent with this model, p53 can both activate and repress a number of viral and cellular promoters. p53 has also been shown to bind to the CCAAT-binding Factor and TATA-binding protein (TBP), and there is direct evidence that p53 represses in vitro transcription by preventing TBP from binding DNA. We now provide evidence that p53 can repress transcription from the SV40 promoter by disrupting DNA/protein complexes involving transcription factor Sp1.
现在有很多证据表明，p53肿瘤抑制蛋白的功能是监测基因组的完整性。当检测到DNA损伤时，p53会抑制细胞生长以进行修复，或引导细胞凋亡。p53的作用机制尚不清楚，但最近积累的证据表明，p53可能通过调节基因表达发挥作用。与该模型一致，p53既能激活也能抑制许多病毒和细胞启动子。p53还被证明能与CCAAT结合因子和TATA结合蛋白（TBP）结合，并且有直接证据表明p53通过阻止TBP与DNA结合来抑制体外转录。我们现在提供证据表明，p53可通过破坏涉及转录因子Sp1的DNA/蛋白质复合物来抑制SV40启动子的转录。
