Loo C Y, Willcox M D, Knox K W
Institute of Dental Research, Surry Hills, Australia.
Oral Microbiol Immunol. 1994 Feb;9(1):12-8. doi: 10.1111/j.1399-302x.1994.tb00208.x.
Twenty-nine strains from the Actinomyces species were tested for a range of surface properties. Results show considerable heterogeneity both between different species and within some of the species, especially Actinomyces naeslundii. Two commonly used A. naeslundii strains, T14V and ATCC 12104, fell within the low (salivary aggregation and collagen binding by T14V), moderate (surface charge and haemagglutination) or high range of values (hydrophobicity, saliva-coated hydroxyapatite adhesion, polystyrene binding by T14V, fibrinogen binding by T14V and collagen binding by A. naeslundii ATCC 12104). Both strains adhered well to saliva-coated hydroxyapatite; T14V bound the highest amount of fibrinogen, ATCC 12104 had the highest number of cells bound to collagen and T14V was not bound at all. The heterogeneity of these characteristics highlights the need to include a range of strains of Actinomyces in studies on their pathogenicity. Statistical correlations were found between a number of properties, for example saliva-coated hydroxyapatite adhesion and hydrophobicity, and between haemagglutination and hydrophobicity.
对29株放线菌的一系列表面特性进行了测试。结果表明，不同物种之间以及某些物种内部，尤其是内氏放线菌，存在相当大的异质性。两种常用的内氏放线菌菌株，T14V和ATCC 12104，处于低值范围（T14V的唾液聚集和胶原结合）、中等值范围（表面电荷和血凝）或高值范围（疏水性、唾液包被的羟基磷灰石黏附、T14V的聚苯乙烯结合、T14V的纤维蛋白原结合以及ATCC 12104的胶原结合）。两种菌株都能很好地黏附于唾液包被的羟基磷灰石；T14V结合的纤维蛋白原量最高，ATCC 12104与胶原结合的细胞数量最多，而T14V根本不与胶原结合。这些特性的异质性凸显了在放线菌致病性研究中纳入一系列菌株的必要性。发现许多特性之间存在统计相关性，例如唾液包被的羟基磷灰石黏附和疏水性之间，以及血凝和疏水性之间。
