Olsvik B, Olsen I, Tenover F C
University of Oslo, Norway.
Oral Microbiol Immunol. 1994 Aug;9(4):251-5. doi: 10.1111/j.1399-302x.1994.tb00067.x.
Twenty-two tetracycline-resistant (tetr) anaerobic and facultative anaerobic bacteria isolated from periodontal pockets of 12 patients with refractory periodontitis were examined for the presence of the Tet Q determinant by DNA-DNA hybridization. Dot blots of bacterial DNA were tested with an intragenic digoxigenin-labelled tet(Q) probe consisting of a 1.45 kb EcoRI/PvuII fragment from plasmid pNFD13-2. Southern blots of chromosomal DNA digested with the restriction enzyme EcoRI were also examined. The tet(Q) probe hybridized with DNA from 8 of the 22 tetr strains, including 2 Prevotella intermedia strains and one strain each of Prevotella nigrescens, Prevotella loescheii, Prevotella veroralis and Prevotella melaninogenica. The tetr strains of Mitsuokella dentalis and Capnocytophaga ochracea also hybridized with the probe. The lack of discernible plasmid DNA in all the probe-positive isolates suggests that these tetracycline-resistance genes were chromosomally encoded. The probe hybridized with a different size fragment in all the isolates. This study extends the number of species that carry the tet(Q) gene to include several outside the genera Prevotella and Bacteroides.
从12例难治性牙周炎患者的牙周袋中分离出22株耐四环素（tetr）厌氧和兼性厌氧细菌，通过DNA-DNA杂交检测Tet Q决定簇的存在。用由质粒pNFD13-2的1.45 kb EcoRI/PvuII片段组成的基因内地高辛标记tet(Q)探针检测细菌DNA的斑点印迹。还用限制性内切酶EcoRI消化的染色体DNA的Southern印迹进行了检测。tet(Q)探针与22株tetr菌株中的8株的DNA杂交，包括2株中间普氏菌菌株以及变黑普氏菌、洛氏普氏菌、口腔普氏菌和产黑色素普氏菌各1株。龋齿梭杆菌和赭色二氧化碳嗜纤维菌的tetr菌株也与该探针杂交。所有探针阳性分离株中均未检测到可识别的质粒DNA，这表明这些四环素抗性基因是由染色体编码的。该探针在所有分离株中与不同大小的片段杂交。本研究扩展了携带tet(Q)基因的物种数量，使其包括普氏菌属和拟杆菌属以外的几个属。
