• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Enhanced direct amplification of Guthrie card DNA following selective elution of PCR inhibitors.

作者信息

Makowski G S, Davis E L, Aslanzadeh J, Hopfer S M

机构信息

Department of Laboratory Medicine, University of Connecticut School of Medicine, Farmington 06030, USA.

出版信息

Nucleic Acids Res. 1995 Sep 25;23(18):3788-9. doi: 10.1093/nar/23.18.3788.

DOI:10.1093/nar/23.18.3788
PMID:7479012
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307281/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c524/307281/cc27b05da676/nar00018-0187-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c524/307281/e02a48f55cbd/nar00018-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c524/307281/e4d5ecc6b09c/nar00018-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c524/307281/cc27b05da676/nar00018-0187-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c524/307281/e02a48f55cbd/nar00018-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c524/307281/e4d5ecc6b09c/nar00018-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c524/307281/cc27b05da676/nar00018-0187-b.jpg

相似文献

1
Enhanced direct amplification of Guthrie card DNA following selective elution of PCR inhibitors.PCR抑制剂选择性洗脱后Guthrie卡DNA的增强直接扩增
Nucleic Acids Res. 1995 Sep 25;23(18):3788-9. doi: 10.1093/nar/23.18.3788.
2
Amplification of Guthrie card DNA: effect of guanidine thiocyanate on binding of natural whole blood PCR inhibitors.格思里卡片DNA的扩增：硫氰酸胍对天然全血PCR抑制剂结合的影响
J Clin Lab Anal. 1997;11(2):87-93. doi: 10.1002/(sici)1098-2825(1997)11:2<87::aid-jcla4>3.0.co;2-h.
3
Single tube multiplex PCR detection of 27 cystic fibrosis mutations and 4 polymorphisms using neonatal blood samples collected on Guthrie cards.使用在格思里卡片上采集的新生儿血样对27种囊性纤维化突变和4种多态性进行单管多重PCR检测。
Ann Clin Lab Sci. 2003 Summer;33(3):243-50.
4
The effect of storage on Guthrie cards: implications for deoxyribonucleic acid amplification.
Ann Clin Lab Sci. 1996 Sep-Oct;26(5):458-69.
5
Polymerase chain reaction amplification of Guthrie card deoxyribonucleic acid: extraction of nucleic acid from filter matrices.格思里卡片脱氧核糖核酸的聚合酶链反应扩增：从滤膜基质中提取核酸。
Ann Clin Lab Sci. 1998 Jul-Aug;28(4):254-9.
6
Rapid DNA extraction method for genetic screening.
Eur J Med Res. 1998 Mar 23;3(3):173-5.
7
Direct polymerase chain reaction from whole blood without DNA isolation.
Ann Clin Biochem. 2000 Sep;37 ( Pt 5):674-80. doi: 10.1258/0004563001899726.
8
Rapid genotyping of transgenic mice using dried blood spots from Guthrie cards for PCR analysis.使用Guthrie卡片上的干血斑对转基因小鼠进行快速基因分型以用于PCR分析。
Biotechniques. 1995 May;18(5):758-60.
9
Improved method for direct PCR amplification from whole blood.从全血中直接进行PCR扩增的改进方法。
Nucleic Acids Res. 1992 Dec 25;20(24):6747. doi: 10.1093/nar/20.24.6747.
10
DNA elution and amplification by polymerase chain reaction from dried blood spots.
Biotechniques. 1992 Nov;13(5):735-7.

引用本文的文献

1
Nested PCR Amplification Secures DNA Template Quality and Quantity in Real-time mCOP-PCR Screening for SMA.巢式PCR扩增可确保实时mCOP-PCR筛查脊髓性肌萎缩症时DNA模板的质量和数量。
Kobe J Med Sci. 2019 Jul 16;65(2):E54-E58.
2
Advances in Directly Amplifying Nucleic Acids from Complex Samples.直接从复杂样本中扩增核酸的进展。
Biosensors (Basel). 2019 Sep 30;9(4):117. doi: 10.3390/bios9040117.
3
Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR.基于超顺磁珠的方法：一种从干血斑（DBS）中有效提取用于诊断性PCR的DNA的方法。

本文引用的文献

1
pH modification to enhance the molecular sieving properties of sodium dodecyl sulfate-10% polyacrylamide gels.调节pH值以增强十二烷基硫酸钠-10%聚丙烯酰胺凝胶的分子筛特性。
Anal Biochem. 1993 Jul;212(1):283-5. doi: 10.1006/abio.1993.1324.
2
Mini-gel PAGE for enhanced resolution of polymerase chain reaction detection of delta F508 deletion in cystic fibrosis.
Clin Chem. 1993 Oct;39(10):2204-5.
3
Guthrie spots for DNA-based carrier testing in cystic fibrosis.
Lancet. 1988 Sep 17;2(8612):693. doi: 10.1016/s0140-6736(88)90512-0.
4
J Clin Diagn Res. 2014 Apr;8(4):FC01-4. doi: 10.7860/JCDR/2014/8171.4226. Epub 2014 Apr 15.
4
Comparative analytical utility of DNA derived from alternative human specimens for molecular autopsy and diagnostics.从其他人体标本中提取的 DNA 在分子尸检和诊断中的比较分析效用。
J Mol Diagn. 2012 Sep;14(5):451-7. doi: 10.1016/j.jmoldx.2012.04.005. Epub 2012 Jul 13.
5
Prenatal origin of childhood AML occurs less frequently than in childhood ALL.儿童急性髓系白血病的产前起源比儿童急性淋巴细胞白血病少见。
BMC Cancer. 2006 Apr 21;6:100. doi: 10.1186/1471-2407-6-100.
6
Decrease of 3243 A-->G mtDNA mutation from blood in MELAS syndrome: a longitudinal study.线粒体脑肌病伴乳酸血症和卒中样发作综合征患者血液中3243A→G线粒体DNA突变的减少：一项纵向研究
Am J Hum Genet. 2001 Jan;68(1):238-40. doi: 10.1086/316930. Epub 2000 Nov 20.
7
Backtracking leukemia to birth: identification of clonotypic gene fusion sequences in neonatal blood spots.将白血病追溯至出生时：新生儿血斑中克隆型基因融合序列的鉴定
Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13950-4. doi: 10.1073/pnas.94.25.13950.
8
Amplification of Guthrie card DNA: effect of guanidine thiocyanate on binding of natural whole blood PCR inhibitors.格思里卡片DNA的扩增：硫氰酸胍对天然全血PCR抑制剂结合的影响
J Clin Lab Anal. 1997;11(2):87-93. doi: 10.1002/(sici)1098-2825(1997)11:2<87::aid-jcla4>3.0.co;2-h.
Molecular genetic diagnosis of sickle cell disease using dried blood specimens on blotters used for newborn screening.使用用于新生儿筛查的吸水纸上的干血标本对镰状细胞病进行分子遗传学诊断。
Hum Genet. 1989 Mar;81(4):363-6. doi: 10.1007/BF00283692.
5
Identification of mutations in regions corresponding to the two putative nucleotide (ATP)-binding folds of the cystic fibrosis gene.在与囊性纤维化基因的两个假定核苷酸（ATP）结合折叠相对应的区域中鉴定突变。
Proc Natl Acad Sci U S A. 1990 Nov;87(21):8447-51. doi: 10.1073/pnas.87.21.8447.
6
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.Chelex 100作为一种从法医材料中简单提取用于基于PCR分型的DNA的介质。
Biotechniques. 1991 Apr;10(4):506-13.
7
Cystic fibrosis genotyping by direct PCR analysis of Guthrie blood spots.
PCR Methods Appl. 1992 Nov;2(2):154-6. doi: 10.1101/gr.2.2.154.