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bZip蛋白C/EBP和GCN4不同DNA结合特异性的比较。

A comparison of the different DNA binding specificities of the bZip proteins C/EBP and GCN4.

作者信息

Koldin B, Suckow M, Seydel A, von Wilcken-Bergmann B, Müller-Hill B

机构信息

Institut für Genetik, Universität zu Köln, Germany.

出版信息

Nucleic Acids Res. 1995 Oct 25;23(20):4162-9. doi: 10.1093/nar/23.20.4162.

DOI:10.1093/nar/23.20.4162
PMID:7479080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307358/
Abstract

The bZip proteins GCN4 and C/EBP differ in their DNA binding specificities: GCN4 binds well to the pseudopalindromic AP1 site 5'-A4T3G2A1C0T1C2'A3'T4'-3' and to the palindromic ATF/CREB sequence 5'-A4T3G2A1-C0G0'T1'C2'A3'T4'-3'; C/EBP preferentially recognizes the palindromic sequence 5'-A4T3T2G1C0G0'C1'A2'-A3'T4'-3'. According to the X-ray structures of GCN4-DNA complexes, five residues of the basic region of GCN4 are involved in specific base contacts: asparagine -18, alanine -15, alanine -14, serine -11 and arginine -10 (numbered relative to the start point of the leucine zipper, which we define as +1). In the basic region of C/EBP position -14 is occupied by valine instead of alanine, the other four residues being identical. Here we analyse the role of valine -14 in C/EBP-DNA complex formation. Starting from a C/EBP-GCN4 chimeric bZip peptide which displays C/EBP specificity, we systematically mutated position -14 of its basic region and characterized the DNA binding specificities of the 20 possible different peptides by gel mobility shift assays with various target sites. We present evidence that valine -14 of C/EBP interacts more strongly with thymine 2 than with cytosine 1' of the C/EBP binding site, unlike the corresponding alanine -14 of GCN4, which exclusively contacts thymine 1' of the GCN4 binding sites.

摘要

碱性亮氨酸拉链（bZip）蛋白GCN4和C/EBP在DNA结合特异性方面存在差异：GCN4能很好地结合假回文AP1位点5'-A4T3G2A1C0T1C2'A3'T4'-3'以及回文ATF/CREB序列5'-A4T3G2A1-C0G0'T1'C2'A3'T4'-3'；C/EBP则优先识别回文序列5'-A4T3T2G1C0G0'C1'A2'-A3'T4'-3'。根据GCN4-DNA复合物的X射线结构，GCN4碱性区域的五个残基参与特定碱基接触：天冬酰胺-18、丙氨酸-15、丙氨酸-14、丝氨酸-11和精氨酸-10（相对于亮氨酸拉链的起始点编号，我们将其定义为+1）。在C/EBP的碱性区域，位置-14由缬氨酸占据而非丙氨酸，其他四个残基相同。在此我们分析缬氨酸-14在C/EBP-DNA复合物形成中的作用。从展示C/EBP特异性的C/EBP-GCN4嵌合bZip肽开始，我们系统地突变其碱性区域的位置-14，并通过对各种靶位点进行凝胶迁移率变动分析来表征20种可能不同肽段的DNA结合特异性。我们提供的证据表明，与GCN4相应的丙氨酸-14专门接触GCN4结合位点的胸腺嘧啶1'不同，C/EBP的缬氨酸-14与C/EBP结合位点的胸腺嘧啶2的相互作用比与胞嘧啶1'的更强。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/951a/307358/a81e2bf4bf88/nar00020-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/951a/307358/a81e2bf4bf88/nar00020-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/951a/307358/a81e2bf4bf88/nar00020-0162-a.jpg

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Genes Dev. 1993 Jun;7(6):1047-58. doi: 10.1101/gad.7.6.1047.
2
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3
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Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6462-7. doi: 10.1073/pnas.94.12.6462.
4
Characterization of a zinc-dependent transcriptional activator from Arabidopsis.拟南芥中一种锌依赖性转录激活因子的特性分析
Nucleic Acids Res. 1996 Dec 1;24(23):4624-31. doi: 10.1093/nar/24.23.4624.
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4
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5
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