Jougasaki M, Wei C M, Heublein D M, Sandberg S M, Burnett J C
Cardiorenal Research Laboratory, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
Peptides. 1995;16(4):773-5. doi: 10.1016/0196-9781(95)00011-8.
Adrenomedullin (ADM) is a new endogenous hypotensive and vasorelaxing peptide that may play an important role in the regulation of cardiovascular function. Although ADM was originally isolated from pheochromocytoma, ADM-like immunoreactivity has also been widely detected in various tissues, including the cardiovascular system. Based upon the reports that ADM mRNA and ADM-like immunoreactivity are present in the heart, the present study was designed to investigate the immunohistochemical localization of ADM in the canine heart and aorta. In the canine heart, immunohistochemical examination revealed positive immunostaining within the myocardia in both atria and ventricles. ADM immunoreactivity was observed within the cytoplasm of myocardium, and was widely distributed in the peripheral cytoplasm. ADM immunoreactivity was more intense in the atria than in the ventricles. In the canine aorta, vascular smooth muscle cells of the aorta and vasa vasorum were also immunopositive for ADM. ADM immunoreactivity was mostly localized in the perinuclear position within the smooth muscle cells. There was no immunoreactivity in endothelium, endocardium, epicardium, adventitia, or connective tissues. The current study demonstrates for the first time that immunoreactive ADM by immunohistochemistry is present in the cardiovascular system. As ADM has hypotensive and vasorelaxing actions and circulates in the body, ADM is a cardiovascular peptide hormone that may play an important role in the regulation of cardiovascular system.
肾上腺髓质素（ADM）是一种新的内源性降压和血管舒张肽，可能在心血管功能调节中发挥重要作用。尽管ADM最初是从嗜铬细胞瘤中分离出来的，但在包括心血管系统在内的各种组织中也广泛检测到了ADM样免疫反应性。基于ADM mRNA和ADM样免疫反应性存在于心脏中的报道，本研究旨在探讨ADM在犬心脏和主动脉中的免疫组织化学定位。在犬心脏中，免疫组织化学检查显示心房和心室的心肌内均有阳性免疫染色。ADM免疫反应性在心肌细胞质内被观察到，并广泛分布于周边细胞质中。ADM免疫反应性在心房中比在心室中更强。在犬主动脉中，主动脉的血管平滑肌细胞和血管滋养管对ADM也呈免疫阳性。ADM免疫反应性大多定位于平滑肌细胞内核周位置。在内皮、心内膜、心外膜、外膜或结缔组织中未发现免疫反应性。本研究首次证明通过免疫组织化学检测到的免疫反应性ADM存在于心血管系统中。由于ADM具有降压和血管舒张作用且在体内循环，ADM是一种心血管肽激素，可能在心血管系统调节中发挥重要作用。
