来自具有多氯联苯（PCB）降解活性的假单胞菌属菌株KKS102的2,3-二羟基联苯双加氧酶的结晶及初步晶体学分析
Crystallization and preliminary crystallographic analysis of a 2,3-dihydroxybiphenyl dioxygenase from Pseudomonas sp. strain KKS102 having polychlorinated biphenyl (PCB)-degrading activity.
作者信息
Sugiyama K, Narita H, Yamamoto T, Senda T, Kimbara K, Inokuchi N, Iwama M, Irie M, Fukuda M, Yano K
机构信息
Department of BioEngineering, Nagaoka University of Technology, Niigata, Japan.
出版信息
Proteins. 1995 Jul;22(3):284-6. doi: 10.1002/prot.340220309.
PMID:7479701
Abstract
Crystals have been obtained for a 2,3-dihydroxybiphenyl dioxygenase (conventionally called BphC) from a polychlorinated biphenyl (PCB)-degrader, Pseudomonas sp. strain KKS102. The crystals were grown using both ammonium sulfate and MPD as the precipitating agents. The crystals belonged to a tetragonal space group (I422) and diffracted to 2.5 A.
摘要
已从多氯联苯（PCB）降解菌假单胞菌属菌株KKS102中获得了2,3-二羟基联苯双加氧酶（通常称为BphC）的晶体。使用硫酸铵和MPD作为沉淀剂培养出了这些晶体。这些晶体属于四方晶系空间群（I422），衍射分辨率达到2.5埃。
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