McPartlin J, Skrabanek P, Powell D
Endocrinology. 1978 Nov;103(5):1573-8. doi: 10.1210/endo-103-5-1573.
Using a quantitative cytochemical method, explanted calvaria of 1- to 6-day-old male rats were used for the measurement of the in situ effect of parathyroid hormone (PTH) on the activity of alkaline phosphatase (AP) in the calcifying bone. The earliest effect of PTH was observed at 4--7 min. The direction and magnitude of the response of AP was dependent on the age of the animal and on the dose of PTH (50 fg-500 pg/ml). The calvaria of younger animals (2-days old) showed a decrease in AP activity, whereas in 5-day-old rats, AP activity was stimulated by PTH. In 5-day-old animals, the linear dependence of ATP stimulation on the PTH dose in the concentration range 50 fg-50 pg/ml could serve as the basis for a bioassay of a high sensitivity. The age-dependent response of AP to PTH may reflect the difference between the early and late stages of the calcification process. Our observations may reconcile conflicting reports on the effect of PTH on bone AP.
采用定量细胞化学方法，以1至6日龄雄性大鼠的颅骨外植体为材料，测定甲状旁腺激素（PTH）对钙化骨中碱性磷酸酶（AP）活性的原位效应。最早在4至7分钟观察到PTH的效应。AP反应的方向和幅度取决于动物的年龄以及PTH的剂量（50飞克至500皮克/毫升）。较年幼动物（2日龄）的颅骨显示AP活性降低，而在5日龄大鼠中，PTH刺激了AP活性。在5日龄动物中，ATP刺激在50飞克至50皮克/毫升浓度范围内对PTH剂量的线性依赖性可作为高灵敏度生物测定的基础。AP对PTH的年龄依赖性反应可能反映了钙化过程早期和晚期的差异。我们的观察结果可能调和了关于PTH对骨AP作用的相互矛盾的报道。
