Ricciardelli E J, Persing J A, Romano J A, Morgan R F, Ogle R C
Section of Plastic Surgery, University of Iowa Hospitals and Clinics, Iowa City, USA.
Plast Reconstr Surg. 1995 Dec;96(7):1689-93. doi: 10.1097/00006534-199512000-00024.
We have studied the chemomigration activity of an epithelial carcinoma cell line using a modified 96-well Boyden chamber apparatus consisting of upper and lower wells separated by an 8-microns pore polycarbonate filter. Cells from the malignant squamous carcinoma cell line A-431 were plated in the upper wells over a collagen IV-coated filter. In chemokinesis assays, the cells were allowed to migrate toward NIH 3T3 fibroblast-conditioned medium or control media in the lower wells for 6 hours at 37 degrees C with 10% CO2. A-431 cells preferentially migrate across the barrier toward conditioned media but not control media. Control normal keratinocytes showed no migration. A highly metastatic melanoma cell line and poorly metastatic melanoma cell line, in which chemomigration has been shown previously to correlate with metastatic potential, were used as positive and negative cellular controls. This system provides a rapidly quantifiable method by which the invasion characteristics of multiple cell lines can be studied simultaneously in a single assay using the 96-well format.
我们使用一种改良的96孔博伊登小室装置研究了一种上皮癌细胞系的化学迁移活性，该装置由上下两个小室组成，中间隔着一个孔径为8微米的聚碳酸酯滤膜。来自恶性鳞状癌细胞系A-431的细胞接种在上部小室中涂有IV型胶原的滤膜上。在化学运动性测定中，让细胞在37℃、10%二氧化碳条件下向下部小室中的NIH 3T3成纤维细胞条件培养基或对照培养基迁移6小时。A-431细胞优先穿过屏障向条件培养基迁移，而不向对照培养基迁移。对照正常角质形成细胞未显示迁移。一种高转移性黑色素瘤细胞系和一种低转移性黑色素瘤细胞系（先前已证明其化学迁移与转移潜能相关）被用作阳性和阴性细胞对照。该系统提供了一种快速可量化的方法，通过该方法可以在单个实验中使用96孔板形式同时研究多个细胞系的侵袭特性。
