Heyer A G, Mozley D, Landschütze V, Thomas B, Gatz C
Institut für Genbiologische Forschung GmbH, Berlin, Germany.
Plant Physiol. 1995 Sep;109(1):53-61. doi: 10.1104/pp.109.1.53.
We have generated transgenic potato plants (Solanum tuberosum) containing the potato phytochrome protein encoded by the PHYA gene cDNA (phyA) in sense or antisense orientation under the control of the 35S cauliflower mosaic virus promoter. Plants with increased and decreased phyA levels were analyzed. When grown under white light, development and growth of sprouts and plants were barely distinguishable from wild type. Under continuous far-red light, stem extension, leaf expansion, and hook opening of sprouts were accelerated in phyA overexpressors and delayed in antisense plants. Sprouts with reduced phyA levels were less sensitive to red light with regard to stem extension and expression of the small subunit genes for ribulose bisphosphate carboxylase. Under low red light:far-red light ratios, increased phyA levels reduced the stem extension component of the shade-avoidance response, whereas decreased levels led to an increase in the response.
我们已经培育出转基因马铃薯植株（茄属马铃薯），其含有由PHYA基因cDNA编码的马铃薯光敏色素蛋白（phyA），该蛋白以正义或反义方向置于35S花椰菜花叶病毒启动子的控制之下。对phyA水平升高和降低的植株进行了分析。在白光下生长时，芽和植株的发育及生长与野生型几乎没有区别。在连续远红光下，phyA过表达植株的茎伸长、叶片扩展和芽的弯钩张开加速，而反义植株则延迟。phyA水平降低的芽在茎伸长和1,5-二磷酸核酮糖羧化酶小亚基基因表达方面对红光的敏感性较低。在低红光：远红光比例下，phyA水平升高会降低避荫反应的茎伸长成分，而phyA水平降低则会导致该反应增强。
