Poppe C, Ehmann B, Frohnmeyer H, Furuya M, Schäfer E
Institut für Biologie II, Universität Freiburg, Germany.
Plant Mol Biol. 1994 Oct;26(1):481-6. doi: 10.1007/BF00039558.
A cDNA clone encoding the apoprotein of a parsley phytochrome was isolated and classified as parsley PHYA phytochrome, on the basis of a sequence homology comparison with all available phytochrome sequences. Red light pulses led to a phytochrome-dependent down-regulation of PHYA mRNA abundance in etiolated parsley seedlings to a level of 10-20% compared with the dark control. The PHYA mRNA abundance in a parsley cell suspension culture was also down-regulated by light pulses. Transient expression assays in parsley protoplasts showed light regulation of a chimeric pea PHYA promoter uidA-gene construct.
通过与所有可得的光敏色素序列进行序列同源性比较，分离出了一个编码欧芹光敏色素脱辅基蛋白的cDNA克隆，并将其归类为欧芹PHYA光敏色素。红光脉冲导致黄化欧芹幼苗中PHYA mRNA丰度出现依赖于光敏色素的下调，与黑暗对照相比下调至10%-20%的水平。光脉冲也下调了欧芹细胞悬浮培养物中PHYA mRNA的丰度。在欧芹原生质体中的瞬时表达分析表明，嵌合豌豆PHYA启动子uidA基因构建体受到光调控。
