Wiebelitz K R, Schrör K
Department of Neonatology, Children's Hospital, University of Tübingen, Germany.
Prostaglandins. 1995 Jun;49(6):323-37. doi: 10.1016/0090-6980(95)00001-q.
The presented study investigates the time-dependent release of PGI2 and TXA2 by isolated human umbilical veins in vitro using the radioimmunoassay for measurement. After changing the nutritional fluid--Krebs-Henseleit solution at pH 7.4, 37 degrees C, 95% O2/5% CO2--the release graph oscillates. These oscillations with time were verified by variance analysis and are very similar for both substances. This indicates one or several negative feedback mechanisms acting on the common path of synthesis from the membrane-bound phospholipids to PGH2, which are effective in the regulation of eicosanoid biosynthesis in vitro. A mathematical function describing the observed PGI2 and TXA2 synthesis is communicated.
本研究采用放射免疫分析法，对离体人脐静脉在体外随时间推移释放前列环素（PGI2）和血栓素A2（TXA2）的情况进行了研究。在更换营养液——pH值为7.4、温度为37℃、含95%氧气/5%二氧化碳的克雷布斯-亨泽莱特溶液后，释放曲线出现振荡。通过方差分析证实了这些随时间的振荡，且两种物质的振荡非常相似。这表明存在一种或几种负反馈机制作用于从膜结合磷脂到前列腺素H2（PGH2）的共同合成途径，这些机制在体外对类花生酸生物合成的调节中发挥作用。文中给出了一个描述观察到的PGI2和TXA2合成的数学函数。
