Ogura A, Yanagimachi R
Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
Reprod Fertil Dev. 1995;7(2):155-8; discussion 158-9. doi: 10.1071/rd9950155.
Intracytoplasmic sperm injection (ICSI) is becoming increasingly popular in human infertility clinics as an efficient method for the treatment of male infertility. It is proposed that spermatids can be used as substitutes for spermatozoa if men are unable to produce sperm in their testes. At least in the hamster and mouse, the nuclei of round spermatids were capable of participating in syngamy when incorporated into homologous mature oocytes either by microsurgical ICSI or electrofusion. Normal mouse offspring were born after after electrofusion of oocytes with round spermatids. When culture in vitro of spermatogonia and spermatocytes is perfected, then spermatids, transforming spermatids and spermatozoa will all be able to be used as male gametes.
胞浆内单精子注射（ICSI）作为治疗男性不育的一种有效方法，在人类不育诊所中越来越受欢迎。有人提出，如果男性睾丸无法产生精子，精子细胞可作为精子的替代品。至少在仓鼠和小鼠中，圆形精子细胞的细胞核通过显微外科ICSI或电融合技术被导入同源成熟卵母细胞后能够参与受精。卵母细胞与圆形精子细胞电融合后产下了正常的小鼠后代。当精原细胞和精母细胞的体外培养完善后，精子细胞、正在转化的精子细胞和精子都将能够用作雄配子。
