Skarsvåg S, Hansen K E, Moen T, Eggen B M
Department of Immunology and Bloodbank, Trondheim Regional Hospital, University of Trondheim, Norway.
Scand J Immunol. 1995 Nov;42(5):564-71. doi: 10.1111/j.1365-3083.1995.tb03697.x.
In order to find potential correlations between HLA class II alleles and anti-SS-A, -SS-B, -Sm and anti-snRNP responses among Norwegian patients with systemic lupus erythematosus (SLE), HLA-DRB1, -DRB30101, -DQA1 and -DQB1 alleles were determined by DNA typing 50 patients and 108 controls. HLA distributions were analysed in the following autoantibody subgroups: anti-SS-A with -SS-B, anti-SS-A without -SS-B, anti-snRNP without -Sm, anti-SS-A without -snRNP and anti-snRNP without -SS-A. The autoantibodies were detected by EIA (enzyme immunuassay). Patients with anti-SS-A and -SS-B had significantly increased frequencies of DRB103, DRB30101, DQA10501, DQB10201 (in linkage disequilibrium) versus controls and versus patients without anti-SS-A and -SS-B. No differences in HLA distribution were found when the group with anti-SS-A alone was compared to the group with anti-SS-A and concomitant -SS-B. Comparing the groups with and without anti-SS-A and -SS-B, the highest RR were found for the alleles DRB103, DRB30101, DQB10501, DQB10201 (in linkage disequilibrium) with RR: 16.8, 5.0, 19.6, 10.3, respectively, P < 0.05). RR for DQw2/DQw6 heterozygotes was 3.5 (Ns.), and RR for cases having DQ alpha molecules with glutamine in position 34 and DQ beta molecules with leucine in position 26 on both chains was 6.3 (P < 0.05). No HLA associations were observed in the group with anti-snRNP without concomitant -Sm or without concomitant -SS-A. These results show that production of anti-SS-A and -SS-B is associated to the HLA alleles DRB103, DRB30101, DQA10501, DQB10201, and that this haplotype shows stronger correlation to these responses than DQw2/DQw6 heterozygosity or HLA molecules having glutamine in position 34 (DQ alpha) and leucine in position 26 (DQ beta). The failure to observe any correlation with DRBI15,16 (DR2) in the group with anti-SS-A alone may demonstrate ethnic differences concerning this response. The failure to identify any HLA associations for the anti-snRNP response may reflect the heterogeneity of the molecules that constitute this antigen.
为了在挪威系统性红斑狼疮（SLE）患者中寻找HLA - II类等位基因与抗SS - A、抗SS - B、抗Sm和抗snRNP反应之间的潜在关联，通过DNA分型对50例患者和108例对照进行了HLA - DRB1、- DRB30101、- DQA1和- DQB1等位基因的检测。在以下自身抗体亚组中分析了HLA分布：抗SS - A伴抗SS - B、抗SS - A不伴抗SS - B、抗snRNP不伴抗Sm、抗SS - A不伴抗snRNP以及抗snRNP不伴抗SS - A。通过酶免疫测定法（EIA）检测自身抗体。与对照组以及不具有抗SS - A和抗SS - B的患者相比，具有抗SS - A和抗SS - B的患者中DRB103、DRB30101、DQA10501、DQB10201（处于连锁不平衡状态）的频率显著增加。将单独具有抗SS - A的组与具有抗SS - A并伴有抗SS - B的组进行比较时，未发现HLA分布存在差异。比较具有和不具有抗SS - A和抗SS - B的组，发现等位基因DRB103、DRB30101、DQB10501、DQB10201（处于连锁不平衡状态）的相对危险度（RR）最高，RR分别为16.8、5.0、19.6、10.3，P < 0.05）。DQw2/DQw6杂合子的RR为3.5（无统计学意义），两条链上第34位为谷氨酰胺的DQα分子和第26位为亮氨酸的DQβ分子的病例的RR为6.3（P < 0.05）。在具有抗snRNP但不伴有抗Sm或不伴有抗SS - A的组中未观察到HLA关联。这些结果表明，抗SS - A和抗SS - B的产生与HLA等位基因DRB103、DRB30101、DQA10501、DQB10201相关，并且该单倍型与这些反应的相关性比DQw2/DQw6杂合性或第34位（DQα）为谷氨酰胺和第26位（DQβ）为亮氨酸的HLA分子更强。在单独具有抗SS - A的组中未观察到与DRBI15,16（DR2）的任何相关性，这可能表明在这种反应方面存在种族差异。未发现抗snRNP反应的任何HLA关联可能反映了构成该抗原的分子的异质性。
