Monostory K, Vereczkey L
Magyar Tudományos Akadémia, Központi Kémiai Kutató Intézet.
Acta Pharm Hung. 1995 Sep;65(5):147-56.
Cytochrome P450 enzymes play essential role in metabolism of exogenous compounds. They are found mainly in endoplasmic reticulum of hepatocytes. Purification of different P450 isoforms and determination of their structures and catalytic activities allowed to investigate their participation in metabolism of xenobiotics. Numerous factors cause variations in the expression of these P450: the effect of genetic and environmental factors results in the production of series of enzymes catalyzing biotransformation of xenobiotics. Several genetic polymorphisms have been described: CYP2D6, CYP2C and 'Ah locus' polymorphism. Some P450 (CYP1A, CYP3A, CYP2E1) are inducible by xenobiotics. Additionally, P450 expression and inducibility vary depending on tissues. It is now possible to determine which isoform(s) is (are) responsible for the production of a metabolite and to predict the fate of a compound in vivo.
细胞色素P450酶在外源化合物的代谢中起着至关重要的作用。它们主要存在于肝细胞的内质网中。不同P450同工型的纯化及其结构和催化活性的测定，有助于研究它们在异生物质代谢中的作用。许多因素导致这些P450表达的变化：遗传和环境因素的影响导致一系列催化异生物质生物转化的酶的产生。已经描述了几种基因多态性：CYP2D6、CYP2C和“A基因座”多态性。一些P450（CYP1A、CYP3A、CYP2E1）可被异生物质诱导。此外，P450的表达和诱导性因组织而异。现在有可能确定哪种同工型负责代谢物的产生，并预测化合物在体内的命运。
