Moore J H, Kolaczynski J W, Morales L M, Considine R V, Pietrzkowski Z, Noto P F, Caro J F
Aesthetic Plast Surg. 1995 Jul-Aug;19(4):335-9. doi: 10.1007/BF00451659.
The results of transplantation of free autologous fat obtained by blunt syringe suction lipectomy are unpredictable. We examined if adipose tissue viability is compromised by using syringe suction lipectomy and by infiltration of the tissue with local anesthetics. As reference, we used adipose tissue samples excised during elective surgery. Fat obtained intraoperatively and by lipectomy was digested with collagenase to isolate adipocytes. The mechanical damage associated with sample handling and cell isolation in both procedures was similar and did not exceed 6% of the total cell mass. In addition, cells isolated from intraoperative and lipectomy samples did not differ functionally, responded similarly to insulin stimulation of glucose transport and epinephrine-stimulated lipolysis, and retained the same growth pattern in culture. Since during fat transplantation the graft is exposed to local anesthetics at both the donor and the recipient sites, we reexamined adipocyte function in the presence of lidocaine. Lidocaine potently inhibited glucose transport and lipolysis in adipocytes and their growth in culture. That effect, however, persisted only as long as lidocaine was present; after washing, the cells were able to fully regain their function and growth regardless of whether the exposure was as short as 30 minutes or as long as 10 days. These results indicate that adipose tissue obtained by syringe lipectomy consists of fully viable and functional adipocytes, but local anesthetics may halt their metabolism and growth.
通过钝头注射器吸脂术获取的自体游离脂肪移植结果是不可预测的。我们研究了使用注射器吸脂术以及用局部麻醉剂浸润组织是否会损害脂肪组织的活力。作为对照，我们使用了择期手术中切除的脂肪组织样本。术中获取的脂肪以及通过吸脂术获得的脂肪用胶原酶消化以分离脂肪细胞。在这两种操作过程中，与样本处理和细胞分离相关的机械损伤相似，且不超过总细胞量的6%。此外，从术中样本和吸脂术样本中分离出的细胞在功能上没有差异，对胰岛素刺激的葡萄糖转运和肾上腺素刺激的脂解反应相似，并且在培养中保持相同的生长模式。由于在脂肪移植过程中，移植物在供体和受体部位都会接触到局部麻醉剂，我们重新研究了利多卡因存在时脂肪细胞的功能。利多卡因能有效抑制脂肪细胞中的葡萄糖转运、脂解及其在培养中的生长。然而，这种作用仅在利多卡因存在时持续；冲洗后，无论暴露时间是短至30分钟还是长达10天，细胞都能够完全恢复其功能和生长。这些结果表明，通过注射器吸脂术获得的脂肪组织由完全有活力且功能正常的脂肪细胞组成，但局部麻醉剂可能会使其代谢和生长停止。
