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Quantitative determination of 8-hydroxyguanine and guanine by isotope dilution mass spectrometry.

作者信息

Hamberg M, Zhang L Y

机构信息

Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.

出版信息

Anal Biochem. 1995 Aug 10;229(2):336-44. doi: 10.1006/abio.1995.1422.

DOI:10.1006/abio.1995.1422
PMID:7485992
Abstract

8-[4,5,6,8-13C4]Hydroxyguanine and [4,5,6-13C3]guanine were prepared by total synthesis. The labeled compounds were used as internal standards in a gas-liquid chromatographic-mass spectrometric method for quantitative determination of 8-hydroxyguanine and guanine in DNA. The approach used made it possible to directly determine the ratio between 8-hydroxyguanine and guanine, expressed as the number of molecules of 8-hydroxyguanine per 10(5) molecules of guanine, in the sample of DNA analyzed. A method for trimethylsilylation of 8-hydroxyguanine and guanine at room temperature was developed. Use of this method for derivatization gave levels of 8-hydroxyguanine in calf thymus DNA which were lower than those recently found by gas chromatography-mass spectrometry but similar to those determined by high-performance liquid chromatography with electrochemical detection.

摘要

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