Brandl R, Maurer P C, Höfling B, Bauriedel G
Department of Vascular Surgery, Technical University of Munich, Germany.
Angiology. 1995 Nov;46(11):973-80. doi: 10.1177/000331979504601101.
Subintimal smooth muscle cell (SMC) migration is considered an essential determinant of arteriosclerosis and neointimal formation. In this study, a cell culture model was established to characterize migration activity of SMCs originating from restenotic and primary lesions. Plaques from symptomatic stenoses of 32 patients (19 men, 13 women; 4 carotid, 17 peripheral, 11 coronary lesions) were removed by percutaneous atherectomy or direct operative approach. Ten patients suffered from recurrent stenosis. Cell cultures were established by explantation of tissue samples. By indirect immunofluorescence microscopy, SMCs were shown to be the predominant cell type of all advanced lesions irrespective of their origin. The spontaneous cellular motility of SMCs was analyzed in vitro by means of a computer-assisted observation system. Cells of all groups exhibited random motility. SMC migratory velocity was found to be significantly (P < 0.001) greater in cells from restenotic lesions than in those from primary plaques. In conclusion, migration behavior of human SMCs originating from arteriosclerotic lesions may be quantified in vitro as a functional determinant characterizing restenotic versus primary lesions.
内膜下平滑肌细胞（SMC）迁移被认为是动脉硬化和新生内膜形成的重要决定因素。在本研究中，建立了一种细胞培养模型来表征源自再狭窄病变和原发性病变的SMC的迁移活性。通过经皮旋切术或直接手术方法，从32例患者（19名男性，13名女性；4例颈动脉病变，17例周围血管病变，11例冠状动脉病变）的症状性狭窄斑块中获取样本。10例患者患有复发性狭窄。通过组织样本植块法建立细胞培养。通过间接免疫荧光显微镜观察，无论起源如何，SMC均为所有晚期病变中的主要细胞类型。利用计算机辅助观察系统在体外分析SMC的自发细胞运动性。所有组的细胞均表现出随机运动。发现再狭窄病变来源的细胞中SMC迁移速度显著高于原发性斑块来源的细胞（P < 0.001）。总之，源自动脉粥样硬化病变的人SMC的迁移行为可在体外进行量化，作为表征再狭窄病变与原发性病变的功能决定因素。
