Nukina N, Uchida K, Sakamoto T, Ide K, Kanazawa I
Department of Neurology, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1995 Nov 13;216(2):467-72. doi: 10.1006/bbrc.1995.2646.
Apolipoprotein E4 has been confirmed as a genetic risk factor for Alzheimer's disease. Although several hypotheses have been advanced to explain how the inheritance of apolipoprotein E isoforms affects the rate of Alzheimer's disease expression, the mechanism whereby apolipoprotein E is involved in the pathogenesis of Alzheimer's disease is still uncertain. To clarify the way in which the apolipoprotein E4 isoform differs from the others, we generated a monoclonal antibody specifically reactive with the apolipoprotein E4 isoform. This antibody suggests that the polymorphic site is important in differentiating the ApoE4 isoform from others.
载脂蛋白E4已被确认为阿尔茨海默病的一个遗传风险因素。尽管已经提出了几种假说来解释载脂蛋白E异构体的遗传如何影响阿尔茨海默病的发病速度，但载脂蛋白E参与阿尔茨海默病发病机制的具体机制仍不明确。为了阐明载脂蛋白E4异构体与其他异构体的不同之处，我们制备了一种与载脂蛋白E4异构体特异性反应的单克隆抗体。该抗体表明，多态性位点对于区分载脂蛋白E4异构体与其他异构体很重要。
