Spencker T, Neumann D, Strasser A, Resch K, Martin M
Institute of Molecular Pharmacology, Medical School Hannover, Germany.
Biochem Biophys Res Commun. 1995 Nov 13;216(2):540-8. doi: 10.1006/bbrc.1995.2656.
The mouse CD5 positive pre-B cell line SPGM-1 can be induced to switch its lineage commitment towards macrophage differentiation by treatment with a combination of phorbol ester and a calcium ionophore. When cultured with these reagents the pre-B cells ceased to proliferate and rapidly became adherent to plastic surfaces. This morphological change was accompanied by the loss of pre-B cell-specific surface markers, such as PB76 and most prominently the mu heavy chain of the immunoglobulin receptor complex. In addition, the mRNA of the surrogate light chain lambda 5 disappeared while the induction of lysozyme mRNA could be detected. Differentiated SPGM-1 cells phagocytosed latex beads and showed nonspecific esterase activity. The high efficiency and speed of differentiation in this cellular system makes SPGM-1 a highly suitable model for studying the phenomenon of lineage switching during hemopoesis.
小鼠CD5阳性前B细胞系SPGM-1可通过佛波酯和钙离子载体联合处理诱导其谱系定向转变为巨噬细胞分化。用这些试剂培养时，前B细胞停止增殖并迅速贴附于塑料表面。这种形态学变化伴随着前B细胞特异性表面标志物的丧失，如PB76，最显著的是免疫球蛋白受体复合物的μ重链。此外，替代轻链λ5的mRNA消失，同时可检测到溶菌酶mRNA的诱导。分化的SPGM-1细胞吞噬乳胶珠并表现出非特异性酯酶活性。该细胞系统中分化的高效率和速度使SPGM-1成为研究造血过程中谱系转换现象的高度合适模型。
