全胃肠外营养诱导酿酒酵母产生热休克蛋白并积累海藻糖

Induction of heat-shock proteins and accumulation of trehalose by TPN in Saccharomyces cerevisiae.

作者信息

Fujita K, Iwahashi H, Kodama O, Komatsu Y

机构信息

United Graduated School, Tokyo University of Agriculture and Technology, Japan.

出版信息

Biochem Biophys Res Commun. 1995 Nov 22;216(3):1041-7. doi: 10.1006/bbrc.1995.2725.

DOI:10.1006/bbrc.1995.2725

PMID:7488177

Abstract

TPN [Tetrachloroisophthalonitrile], a kind of disinfectant, affected growth in Saccharomyces cerevisiae. Exposed to TPN under no lethal conditions, 70-, 90-kDa protein and hsp104 were induced. Each of them was not uniformly induced; namely, the 70-kDa protein was more sensitive to TPN among other proteins. Trehalose was also accumulated depending on the concentration. The degree of thermotolerance in yeast cells pretreated with 1.0 mg/l TPN was about 100-fold greater than in the control. Under this condition, TPN-inducible proteins were synthesized but trehalose was not accumulated. Although TPN-inducible proteins and trehalose were definitely synthesized with 10.0 or 100.0mg/l TPN treatment, thermotolerance was not acquired.

摘要

四氯间苯二腈（TPN）是一种消毒剂，会影响酿酒酵母的生长。在非致死条件下暴露于TPN时，会诱导产生70 kDa、90 kDa的蛋白质以及hsp104。它们各自的诱导情况并不一致；也就是说，70 kDa的蛋白质在其他蛋白质中对TPN更为敏感。海藻糖也会根据浓度而积累。用1.0 mg/l TPN预处理的酵母细胞的耐热程度比对照高约100倍。在此条件下，会合成TPN诱导蛋白，但不会积累海藻糖。尽管用10.0或100.0 mg/l TPN处理时肯定会合成TPN诱导蛋白和海藻糖，但并未获得耐热性。