Boldyrev A A, Lopina O D, Kenney M, Johnson P
Department of Biochemistry, Moscow State University, Russia.
Biochem Biophys Res Commun. 1995 Nov 22;216(3):1048-53. doi: 10.1006/bbrc.1995.2726.
The N-terminal sequences of the alpha and beta subunits from the Na/K-ATPase of duck salt gland have been determined by automated Edman degradation chemistry. These sequences were compared to sequences previously reported for Na/K-ATPase subunits from other sources in order to determine the subunit isoform composition of the salt gland enzyme. The comparisons indicate that the duck salt gland enzyme is composed of an alpha-1 subunit and a beta-1 subunit. This subunit isoform composition is consistent with the involvement of this enzyme in sodium excretion as Na/K-ATPases in other tissues involved in sodium excretion also have this subunit isoform composition.
利用自动Edman降解化学法测定了鸭盐腺Na/K-ATP酶α和β亚基的N端序列。将这些序列与先前报道的来自其他来源的Na/K-ATP酶亚基序列进行比较，以确定盐腺酶的亚基同工型组成。比较结果表明，鸭盐腺酶由一个α-1亚基和一个β-1亚基组成。这种亚基同工型组成与该酶参与钠排泄一致，因为参与钠排泄的其他组织中的Na/K-ATP酶也具有这种亚基同工型组成。
