Cooper M S
Department of Zoology, University of Washington, Seattle 98195, USA.
Bioelectromagnetics. 1995;16(4):255-62. doi: 10.1002/bem.2250160408.
Pulsed electric fields directly influence the electrophysiology of tissue cells by transiently perturbing their transmembrane potential. To determine the magnitude and time course of this interaction, electrotonic cable theory was used to calculate the membrane potential perturbations induced in tissue cells by a spatially uniform, pulsed electric field. Analytic solutions were obtained that predict shifts in membrane potential along the length of cells as a function of time in response to an electrical pulse. For elongated tissue cells, or groups of tissue cells that are coupled electrotonically by gap junctions, significant hyperpolarizations and depolarizations can result from millisecond applications of electric fields with strengths on the order of 10-100 mV/cm. The results illustrate the importance of considering cellular cable parameters in assessing the effects of transient electric fields on biological systems, as well as in predicting the efficacy of pulsed electric fields in medical treatments.
脉冲电场通过瞬时扰动组织细胞的跨膜电位直接影响其电生理学。为了确定这种相互作用的大小和时间进程，利用电紧张电缆理论计算由空间均匀的脉冲电场在组织细胞中引起的膜电位扰动。获得了解析解，其预测了响应电脉冲时沿细胞长度的膜电位变化随时间的函数关系。对于细长的组织细胞或通过间隙连接电耦合的组织细胞群，施加强度约为10 - 100 mV/cm的电场毫秒级时间可导致显著的超极化和去极化。结果说明了在评估瞬态电场对生物系统的影响以及预测脉冲电场在医学治疗中的疗效时考虑细胞电缆参数的重要性。
