Power J M, Ringo J M, Dowse H B
Department of Zoology, University of Maine, Crono 04469, USA.
J Biol Rhythms. 1995 Sep;10(3):267-80. doi: 10.1177/074873049501000309.
Strains of Drosophila melanogaster homozygous for alleles of the period gene (perO, perL, perS, and per+) were reared for multiple generations either in light:dark cycles (LD), continuous illumination (LL), or chronic darkness (DD). The locomotor activity of adult flies from these cultures was monitored in either LL or DD. Flies that were reared and tested in DD had a lower proportion of individuals with normal circadian rhythms than flies reared in LD or LL and tested in DD. The activity rhythms of DD-reared DD-tested animals, when present, showed phase coherence within two out of seven populations, while 8 out of 10 LL-reared DD-tested showed phase coherence. Flies tested in LL were largely devoid of circadian rhythms regardless of their rearing environment. Ultradian rhythms were more evident under conditions disruptive to circadian rhythmicity, but were observed in the presence and absence of circadian rhythms. The periods of the ultradian rhythms of LL-reared DD-tested and LD-reared DD-tested flies varied significantly among genotypes, while in other rearing and testing regimes, no relationship was found.
将周期基因（perO、perL、perS和per+）等位基因纯合的黑腹果蝇品系在明:暗周期（LD）、持续光照（LL）或长期黑暗（DD）条件下饲养多代。从这些培养物中羽化出的成年果蝇的运动活动在LL或DD条件下进行监测。与在LD或LL条件下饲养并在DD条件下测试的果蝇相比，在DD条件下饲养并测试的果蝇中具有正常昼夜节律的个体比例较低。当存在时，在DD条件下饲养并在DD条件下测试的动物的活动节律在七个种群中的两个种群内表现出相位一致性，而在LL条件下饲养并在DD条件下测试的果蝇中，十分之八表现出相位一致性。无论饲养环境如何，在LL条件下测试的果蝇基本没有昼夜节律。超日节律在破坏昼夜节律性的条件下更为明显，但在有和没有昼夜节律的情况下均能观察到。在LL条件下饲养并在DD条件下测试的果蝇以及在LD条件下饲养并在DD条件下测试的果蝇的超日节律周期在不同基因型之间有显著差异，而在其他饲养和测试条件下，则未发现相关性。
