Moldenhauer J E, Rubio S L, Pflug I J
Fujisawa USA, Inc., Deerfield, Illinois, USA.
PDA J Pharm Sci Technol. 1995 Sep-Oct;49(5):235-8.
Bacillus coagulans, ATCC 51232 (formerly referred to as FRR B666), was previously reported to be a potentially excellent biological indicator. The organism was grown on a relatively simple sporulation media and the spores were evaluated for moist heat resistance in various parenteral solutions. Resistance was determined using the survivor curve method. For those solutions which appeared to be inhibitory to the outgrowth of Bacillus coagulans, ATCC 51232, further investigations were performed to evaluate the inhibitory effect. The results obtained demonstrated that the organism had the highest resistance in Calcium Gluconate Injection; USP (100 mg/mL) at 120 degrees C.
凝结芽孢杆菌ATCC 51232（以前称为FRR B666），先前被报道为一种潜在的优良生物指示剂。该菌株在相对简单的芽孢形成培养基上生长，并在各种注射用溶液中评估其孢子的湿热耐受性。使用存活曲线法测定耐受性。对于那些似乎对凝结芽孢杆菌ATCC 51232生长有抑制作用的溶液，进行了进一步研究以评估抑制效果。所获得的结果表明，该菌株在120℃的葡萄糖酸钙注射液USP（100mg/mL）中具有最高的耐受性。
