当细胞失去正常功能时：无义密码子对脊椎动物细胞RNA代谢的影响。
When cells stop making sense: effects of nonsense codons on RNA metabolism in vertebrate cells.
作者信息
Maquat L E
机构信息
Department of Human Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
出版信息
RNA. 1995 Jul;1(5):453-65.
Abstract
It appears that no organism is immune to the effects of nonsense codons on mRNA abundance. The study of how nonsense codons alter RNA metabolism is still at an early stage, and our current understanding derives more from incidental vignettes than from experimental undertakings that address molecular mechanisms. Challenges for the future include identifying the gene products and RNA sequences that function in nonsense mediated RNA loss, resolving the cause and consequences of there apparently being more than one cellular site and mechanism for nonsense-mediated RNA loss, and understanding how these sites and mechanisms are related to both constitutive and specialized pathways of pre-mRNA processing and mRNA decay.
摘要
似乎没有一种生物体对无义密码子对mRNA丰度的影响具有免疫力。关于无义密码子如何改变RNA代谢的研究仍处于早期阶段，我们目前的理解更多地来自偶然的事例，而非来自针对分子机制的实验研究。未来的挑战包括确定在无义介导的RNA降解中起作用的基因产物和RNA序列，解决无义介导的RNA降解明显存在多个细胞位点和机制的原因及后果，以及了解这些位点和机制如何与前体mRNA加工和mRNA降解的组成型及特殊途径相关。
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