Gilfillan S, Bachmann M, Trembleau S, Adorini L, Kalinke U, Zinkernagel R, Benoist C, Mathis D
Institut de Génétique et de Biologie Moléculaire et Cellulaire, (INSERM/CNRD/ULP) Illkirch, C. U. de Strasbourg, France.
Eur J Immunol. 1995 Nov;25(11):3115-22. doi: 10.1002/eji.1830251119.
Mice with a null mutation in the terminal deoxynucleotidyl transferase (TdT) gene harbor immunoglobulin and T cell receptor repertoires essentially devoid of N-region diversity. Consequently, the CDR3 loops important for antigen recognition are shorter and considerably less diverse than those of wild-type controls. We find surprisingly normal immune responses in TdT0 mice, as regards both efficiency and specificity. This provokes a reconsideration of the assumption that N-region diversity is required for an effective T and B cell repertoire.
末端脱氧核苷酸转移酶（TdT）基因发生无效突变的小鼠，其免疫球蛋白和T细胞受体库基本没有N区多样性。因此，对抗原识别很重要的互补决定区3（CDR3）环比野生型对照的更短且多样性显著更低。我们发现，在TdT0小鼠中，无论是在效率还是特异性方面，免疫反应都出人意料地正常。这引发了人们对有效T和B细胞库需要N区多样性这一假设的重新思考。
