Fiedorek F T, Kay E S
Department of Medicine and Nutrition, University of North Carolina School of Medicine, Chapel Hill 27599, USA.
Genomics. 1995 Aug 10;28(3):581-4. doi: 10.1006/geno.1995.1193.
We have identified sequence variants in the 3' untranslated region of the insulin promoter factor 1 (Ipf1) cDNA sequence in mice and used a PCR-based oligonucleotide hybridization assay to map the Ipf1 gene to distal mouse chromosome (Chr) 5. An identical 12-bp insertion in the 3' untranslated region of the mouse Ipf1 gene sequence is present in Mus spretus and Mus m. musculus Czech II mice, two feral strains used for meiotic mapping by backcross analysis. The human IPF1 gene homolog would be expected to map to either chromosome 13q12-q14 or 7pter-q21 based on homology of synteny of other loci in this region of mouse Chr 5. Given its genetic map position, the Ipf1 gene may be part of a cassette of homeodomain-containing transcription factors involved in the development of the pancreas and other foregut-derived organs.
我们已经在小鼠胰岛素启动子因子1（Ipf1）cDNA序列的3'非翻译区鉴定出序列变异，并使用基于PCR的寡核苷酸杂交测定法将Ipf1基因定位到小鼠5号远端染色体（Chr）上。小家鼠和小家鼠捷克II小鼠（两种用于回交分析进行减数分裂定位的野生菌株）的小鼠Ipf1基因序列的3'非翻译区存在相同的12碱基插入。基于小鼠Chr 5该区域其他位点的同线性同源性，预计人类IPF1基因同源物将定位于染色体13q12-q14或7pter-q21。鉴于其遗传图谱位置，Ipf1基因可能是参与胰腺和其他前肠衍生器官发育的含同源结构域转录因子盒的一部分。
