Walker M M, Smolka A, Waller J M, Evans D J
Department of Histopathology, St Mary's Hospital Medical School, London.
J Clin Pathol. 1995 Sep;48(9):832-4. doi: 10.1136/jcp.48.9.832.
To identify parietal cells in the upper gastrointestinal tract by an immunoperoxidase method, using commercially available monoclonal antibodies.
Routine surgical biopsy specimens of gastric body mucosa were examined using the avidin-biotin peroxidase method with the monoclonal antibodies HMFG-1 and HMFG-2 to identify parietal cells. Double immunoperoxidase labelling with HK12.18, a well characterised monoclonal antibody directed against an epitope on the alpha (catalytic) subunit of H+ translocating, K+ stimulated adenosine triphosphatase (H,K-ATPase), was used to confirm that HMFG-1 and -2 stained parietal cells.
HMFG-1 and HMFG-2 showed consistent parietal cell staining patterns in the gastric body mucosa. HMFG-2 gave a more intense staining pattern of the secretory canaliculi. This was confirmed by double immunolabelling with HK12.18.
HMFG monoclonal antibodies are recommended as highly specific markers of human gastric parietal cells.
使用市售单克隆抗体，通过免疫过氧化物酶法鉴定上消化道中的壁细胞。
采用抗生物素蛋白-生物素过氧化物酶法，用单克隆抗体HMFG-1和HMFG-2检测胃体黏膜的常规手术活检标本，以鉴定壁细胞。用HK12.18进行双重免疫过氧化物酶标记，HK12.18是一种针对H⁺转运、K⁺刺激的三磷酸腺苷酶（H，K-ATP酶）α（催化）亚基上一个表位的特征明确的单克隆抗体，用于确认HMFG-1和-2对壁细胞的染色。
HMFG-1和HMFG-2在胃体黏膜中显示出一致的壁细胞染色模式。HMFG-2对分泌小管的染色更强。用HK12.18进行双重免疫标记证实了这一点。
推荐HMFG单克隆抗体作为人胃壁细胞的高度特异性标志物。
