Keränen U, Vanhatalo S, Kiviluoto T, Kivilaakso E, Soinila S
Second Department of Surgery, Helsinki University Central Hospital, Finland.
J Auton Nerv Syst. 1995 Sep 5;54(3):177-83. doi: 10.1016/0165-1838(95)00010-u.
Nitric oxide-containing nervous structures were localized in the human colon using NADPH diaphorase activity and nitric oxide synthase immunoreactivity. We found some, solitary NADPH diaphorase-reactive and nitric oxide synthase-immunoreactive neurons in the submucous plexus, while the myenteric plexus contained several neurons, often arranged in clusters, and nerve fibers showing these markers. The circular muscle layer contained a dense plexus of NADPH diaphorase-reactive nerves, which was greater than that in the longitudinal muscle layer. We report on co-localization of NADPH diaphorase activity and VIP immunoreactivity in several neurons of the myenteric ganglia. Such co-localization has not been reported previously for human colon. Localization of nitric oxide synthase and VIP in the myenteric plexus and in the nerves of circular muscle layer raises the possibility that nitric oxide contributes to the regulation of motility in the human colon.
利用还原型辅酶Ⅱ黄递酶活性和一氧化氮合酶免疫反应性，对含一氧化氮的神经结构在人类结肠中的位置进行了定位。我们在黏膜下神经丛中发现了一些单个的还原型辅酶Ⅱ黄递酶反应性和一氧化氮合酶免疫反应性神经元，而肌间神经丛中有多个神经元，常成簇排列，还有显示这些标志物的神经纤维。环形肌层含有密集的还原型辅酶Ⅱ黄递酶反应性神经丛，其比纵行肌层中的更密集。我们报告了在肌间神经节的几个神经元中还原型辅酶Ⅱ黄递酶活性和血管活性肠肽免疫反应性的共定位情况。这种共定位情况此前在人类结肠中尚未见报道。一氧化氮合酶和血管活性肠肽在肌间神经丛以及环形肌层神经中的定位，增加了一氧化氮参与人类结肠运动调节的可能性。
