T细胞受体β链互补决定区3（CDR3）区域的家族内片段分析

Intrafamily fragment analysis of the T cell receptor beta chain CDR3 region.

作者信息

Liu D, Callahan J P, Dau P C

机构信息

Department of Medicine, Evanston Hospital, IL 60201, USA.

出版信息

J Immunol Methods. 1995 Nov 16;187(1):139-50. doi: 10.1016/0022-1759(95)00178-d.

DOI:10.1016/0022-1759(95)00178-d

PMID:7490450

Abstract

The length distributions of the third complementarity determining region (CDR3) of the T cell receptor (TCR) beta chain were determined quantitatively from peripheral blood lymphocytes of healthy donors. RT-PCR products of 26 V beta families and subfamilies were analyzed by an A.L.F. DNA sequencer and Fragment Manager software. We established a normal reference of CDR3 lengths for most of the V beta families and subfamilies. The known range of CDR3 lengths for the V beta chain was expanded to 24 amino acids, and quantitative measurements were made for each fragment length allowing intrafamily CDR3 fragment length comparisons. Importantly, we were able to analyze intrafamily CDR3 fragment distribution without optimizing PCR conditions, thereby circumventing a major obstacle found in intrafamily TCR beta chain comparisons.

摘要

从健康供体的外周血淋巴细胞中定量测定了T细胞受体（TCR）β链第三互补决定区（CDR3）的长度分布。通过A.L.F. DNA测序仪和片段管理软件分析了26个Vβ家族和亚家族的逆转录聚合酶链反应（RT-PCR）产物。我们为大多数Vβ家族和亚家族建立了CDR3长度的正常参考值。Vβ链已知的CDR3长度范围扩大到了24个氨基酸，并对每个片段长度进行了定量测量，以便进行家族内CDR3片段长度比较。重要的是，我们能够在不优化PCR条件的情况下分析家族内CDR3片段分布，从而克服了家族内TCRβ链比较中发现的一个主要障碍。