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来自轮藻属藻类的肌球蛋白:电子显微镜揭示的独特结构。

Myosin from alga Chara: unique structure revealed by electron microscopy.

作者信息

Yamamoto K, Kikuyama M, Sutoh-Yamamoto N, Kamitsubo E, Katayama E

机构信息

Department of Biology Faculty of Science, Chiba University, Japan.

出版信息

J Mol Biol. 1995 Nov 24;254(2):109-12. doi: 10.1006/jmbi.1995.0603.

DOI:10.1006/jmbi.1995.0603
PMID:7490735
Abstract

Cytoplasmic streaming is a fundamental process for the transport of molecules and organelles in plant cells. A myosin-like protein responsible for cytoplasmic streaming in fresh water alga, Chara corallina, has been purified. Electron micrographs of this protein revealed that it has two heads with similar size and shape to those of muscle myosin (myosin II) but its tail was shorter than that of muscle myosin with a globular structure at its distal end like that of myosin V. The myosin cosedimented with vesicles composed of phosphatidyl serine like other non-muscle myosin species involved in intracellular transport.

摘要

胞质环流是植物细胞中分子和细胞器运输的基本过程。一种负责淡水藻类轮藻胞质环流的类肌球蛋白蛋白已被纯化。这种蛋白质的电子显微镜照片显示,它有两个头部,其大小和形状与肌肉肌球蛋白(肌球蛋白II)的相似,但它的尾部比肌肉肌球蛋白的短,在其远端有一个球状结构,类似于肌球蛋白V。与参与细胞内运输的其他非肌肉肌球蛋白种类一样,这种肌球蛋白与由磷脂酰丝氨酸组成的囊泡共沉降。

相似文献

1
Myosin from alga Chara: unique structure revealed by electron microscopy.来自轮藻属藻类的肌球蛋白:电子显微镜揭示的独特结构。
J Mol Biol. 1995 Nov 24;254(2):109-12. doi: 10.1006/jmbi.1995.0603.
2
Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina.蛋白质磷酸化调节从绿藻轮藻中分离出的细胞提取物中肌动球蛋白驱动的囊泡运动。
Cell Motil Cytoskeleton. 2002 Sep;53(1):66-76. doi: 10.1002/cm.10054.
3
Binding of chara Myosin globular tail domain to phospholipid vesicles.轮藻肌球蛋白球状尾部结构域与磷脂囊泡的结合。
Plant Cell Physiol. 2007 Nov;48(11):1558-66. doi: 10.1093/pcp/pcm126. Epub 2007 Oct 4.
4
The molecular structure of the fastest myosin from green algae, Chara.来自绿藻轮藻的最快肌球蛋白的分子结构。
Biochem Biophys Res Commun. 2000 Apr 2;270(1):147-52. doi: 10.1006/bbrc.2000.2391.
5
Partial characterization of a putative 110 kDa myosin from the green alga Chara corallina by in vitro binding of fluorescent F-actin.通过荧光F-肌动蛋白的体外结合对来自绿藻轮藻的一种假定的110 kDa肌球蛋白进行部分表征。
Cell Biol Int. 1996 May;20(5):365-73. doi: 10.1006/cbir.1996.0043.
6
Myosin and Ca2+-sensitive streaming in the alga Chara: detection of two polypeptides reacting with a monoclonal anti-myosin and their localization in the streaming endoplasm.轮藻中的肌球蛋白与钙离子敏感的胞质环流:两种与单克隆抗肌球蛋白反应的多肽的检测及其在流动内质中的定位
Eur J Cell Biol. 1988 Oct;47(1):22-31.
7
The motility of Chara corallina myosin was inhibited reversibly by 2,3-butanedione monoxime (BDM).2,3-丁二酮一肟(BDM)可可逆地抑制珊瑚轮藻肌球蛋白的运动性。
Plant Cell Physiol. 2004 Sep;45(9):1342-5. doi: 10.1093/pcp/pch154.
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Cell and molecular biology of the fastest myosins.最快肌球蛋白的细胞与分子生物学
Int Rev Cell Mol Biol. 2009;276:301-47. doi: 10.1016/S1937-6448(09)76007-1.
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Chara myosin and the energy of cytoplasmic streaming.轮藻肌球蛋白与胞质环流的能量
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Two-headed binding of a processive myosin to F-actin.持续性肌球蛋白与F-肌动蛋白的双头结合。
Nature. 2000 Jun 15;405(6788):804-7. doi: 10.1038/35015592.

引用本文的文献

1
Head-neck domain of Arabidopsis myosin XI, MYA2, fused with GFP produces F-actin patterns that coincide with fast organelle streaming in different plant cells.拟南芥肌球蛋白XI的头部-颈部结构域MYA2与绿色荧光蛋白(GFP)融合后,产生的丝状肌动蛋白(F-肌动蛋白)模式与不同植物细胞中快速的细胞器流动相一致。
BMC Plant Biol. 2008 Jul 3;8:74. doi: 10.1186/1471-2229-8-74.
2
Truncated myosin XI tail fusions inhibit peroxisome, Golgi, and mitochondrial movement in tobacco leaf epidermal cells: a genetic tool for the next generation.截短的肌球蛋白XI尾部融合蛋白抑制烟草叶片表皮细胞中的过氧化物酶体、高尔基体和线粒体运动:一种新一代的遗传工具。
J Exp Bot. 2008;59(9):2499-512. doi: 10.1093/jxb/ern114. Epub 2008 May 23.
3
The sliding theory of cytoplasmic streaming: fifty years of progress.
细胞质环流的滑动理论:五十年的进展
J Plant Res. 2007 Jan;120(1):31-43. doi: 10.1007/s10265-006-0061-0. Epub 2007 Jan 25.
4
Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction.从新月藻(Closterium peracerosum-strigosum-littorale复合体)中分离肌球蛋白XI基因并分析其在有性生殖过程中的表达
J Plant Res. 2006 Mar;119(2):105-13. doi: 10.1007/s10265-005-0249-8. Epub 2006 Feb 3.
5
Higher plant myosin XI moves processively on actin with 35 nm steps at high velocity.高等植物肌球蛋白XI在肌动蛋白上以35纳米的步幅高速连续移动。
EMBO J. 2003 Mar 17;22(6):1263-72. doi: 10.1093/emboj/cdg130.
6
Analysis of the myosins encoded in the recently completed Arabidopsis thaliana genome sequence.对最近完成的拟南芥基因组序列中编码的肌球蛋白的分析。
Genome Biol. 2001;2(7):RESEARCH0024. doi: 10.1186/gb-2001-2-7-research0024. Epub 2001 Jul 3.
7
Biochemical and immunocytochemical characterization of two types of myosins in cultured tobacco bright yellow-2 cells.培养的烟草亮黄-2细胞中两种肌球蛋白的生化和免疫细胞化学特性
Plant Physiol. 1999 Oct;121(2):525-34. doi: 10.1104/pp.121.2.525.
8
A myosin family reunion.肌球蛋白家族团聚。
J Muscle Res Cell Motil. 1996 Feb;17(1):7-22. doi: 10.1007/BF00140320.