Characterisation of the antibodies detected by the film inhibition test for Mycoplasma bovis.

The class of antibody detected by the film inhibition test was determined. Density gradient ultracentrifugation and disulphide-bond reduction showed it to be 7S(IgG) immunoglobulin. Antibody absorption and ion exchange chromatography studies confirmed this and indicated that IgG2 is probably the major type of antibody involved.