Gao J Y, Liu S, Zhang X
Department of Nephrology, Xijing Hospital, Xian, PR of China.
Tissue Cell. 1995 Oct;27(5):499-503. doi: 10.1016/s0040-8166(05)80058-8.
This study was conducted using immunohistochemistry to detect the expression of the prooncogen c-fos in the corticoadrenal of the rat with renal hypertension. A model of renal hypertension was established through narrowing a renal artery of the rat. An antiserum against FOS oncoprotein was used to immunohistochemically detect the activated cells of the corticoadrenal. In the experimental group, C-FOS-immunoreactive (ir) nuclei proved to be present in the zona glomerulosa. Few labeled nuclei were seen in the lateral part of the zona fasciculata. An increasing number of C-FOS-ir nuclei was demonstrated in the intermediate part of the zona fasciculata. The highest concentration of the labeled nuclei was found in the inner part of the zona fasciculata. Scattered FOS-ir nuclei existed in the zona reticularis. The labeled nuclei were often round or oval in shape, and different in size. Only were a few labeled nuclei seen in the sham-operated group and no FOS-ir nuclei in normal control group. The result suggested that all three zones of the corticoadreal were modified subsequent to induction of renal hypertension.
本研究采用免疫组织化学方法检测肾性高血压大鼠肾上腺皮质中癌基因c-fos的表达。通过缩窄大鼠肾动脉建立肾性高血压模型。用抗FOS癌蛋白抗血清对肾上腺皮质激活细胞进行免疫组织化学检测。在实验组中，球状带可见C-FOS免疫反应性（ir）细胞核。束状带外侧可见少量标记细胞核。在束状带中间部分，C-FOS-ir细胞核数量逐渐增多。标记细胞核浓度最高的部位在束状带内侧。网状带存在散在的FOS-ir细胞核。标记细胞核常呈圆形或椭圆形，大小不一。假手术组仅见少量标记细胞核，正常对照组未见FOS-ir细胞核。结果提示，肾性高血压诱导后肾上腺皮质的三个带均发生了改变。
