Salomon R, Bernardi F
Department of Virology, Volcani Center, Bet Dagan, Israel.
Virology. 1995 Nov 10;213(2):676-9. doi: 10.1006/viro.1995.9961.
Since removal of the exposed N-terminus of the coat protein of some potyviruses abolishes aphid transmission, the role of this coat protein region of maize dwarf mosaic potyvirus (MDMV) in aphid transmission was investigated. The viral cDNA encoding this region was cloned and expressed as a fusion protein in bacteria. The resulting purified N-terminus of the coat protein was used in controlled aphid transmission experiments in competition with MDMV. The results show that this region inhibits aphid transmission of MDMV, indicating a direct involvement of the N-terminal region of the coat protein in aphid transmission.
由于去除某些马铃薯Y病毒属病毒外壳蛋白暴露的N端会消除蚜虫传播，因此研究了玉米矮花叶马铃薯Y病毒（MDMV）外壳蛋白的这一区域在蚜虫传播中的作用。编码该区域的病毒cDNA被克隆并在细菌中作为融合蛋白表达。所得纯化的外壳蛋白N端用于与MDMV竞争的可控蚜虫传播实验。结果表明，该区域抑制MDMV的蚜虫传播，表明外壳蛋白的N端区域直接参与蚜虫传播。
